6JP6
The X-ray structure of yeast tRNA methyltransferase complex of Trm7 and Trm734 essential for 2'-O-methylation at the first position of anticodon in specific tRNAs
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL38B1 |
| Synchrotron site | SPring-8 |
| Beamline | BL38B1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2014-06-11 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 1.0 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 253.643, 110.351, 133.503 |
| Unit cell angles | 90.00, 90.02, 90.00 |
Refinement procedure
| Resolution | 36.433 - 2.699 |
| R-factor | 0.1962 |
| Rwork | 0.194 |
| R-free | 0.23870 |
| Structure solution method | SAD |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.227 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | PHENIX |
| Refinement software | PHENIX (1.10.1_2155) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 36.433 | 2.750 |
| High resolution limit [Å] | 2.699 | 2.700 |
| Rmerge | 0.087 | 0.465 |
| Number of reflections | 100973 | 4959 |
| <I/σ(I)> | 22.2 | |
| Completeness [%] | 99.9 | |
| Redundancy | 4.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 293 | PEG400, HEPES-NaOH, ammonium sulfate, phenol, ethylene glycol |
