6JOU

Crystal structure of the human nucleosome containing H2A.Z.1 S42R

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SPRING-8 BEAMLINE BL41XU
Synchrotron site	SPring-8
Beamline	BL41XU
Temperature [K]	100
Detector technology	PIXEL
Collection date	2016-10-31
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	1.000
Spacegroup name	P 21 21 21
Unit cell lengths	98.955, 108.170, 169.812
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	49.477 - 2.170
R-factor	0.2088
Rwork	0.207
R-free	0.25000
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3wa9
RMSD bond length	0.008
RMSD bond angle	1.026
Data scaling software	HKL-2000 (v712)
Phasing software	PHASER (2.7.17)
Refinement software	PHENIX (1.11.1_2575)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	2.250
High resolution limit [Å]	2.170	4.670	2.170
Rmerge	0.083	0.067	0.475
Rmeas	0.087	0.070	0.515
Rpim	0.026	0.021	0.190
Number of reflections	95890
<I/σ(I)>	8.2
Completeness [%]	98.6	99.2	90.9
Redundancy	10.1	10.9	6.2
CC(1/2)		0.997	0.863

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6	293	potassium cacodylate, potassium chloride, manganese chloride