6JO7

Crystal structure of mouse MXRA8

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL17U1
Synchrotron site	SSRF
Beamline	BL17U1
Temperature [K]	100
Detector technology	CCD
Collection date	2018-08-02
Detector	ADSC QUANTUM 315
Wavelength(s)	0.97894
Spacegroup name	I 2 2 2
Unit cell lengths	72.446, 143.486, 196.346
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	49.341 - 2.400
R-factor	0.22
Rwork	0.219
R-free	0.24460
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3tt3
RMSD bond length	0.002
RMSD bond angle	0.606
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX ((1.12_2829: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.490
High resolution limit [Å]	2.400	2.400
Number of reflections	40460	3978
<I/σ(I)>	22.1	1.1
Completeness [%]	100.0	100
Redundancy	13.2	12.3
CC(1/2)	0.997	0.691

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	8.5	291	0.1 M BICINE pH 8.5, 15% w/v Polyethylene glycol 1500