6JO3

Crystal structure of (S)-3-O-geranylgeranylglyceryl phosphate synthase from Thermoplasma acidophilum in complex with substrate sn-glycerol-1-phosphate

Replaces:  5B69

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PHOTON FACTORY BEAMLINE AR-NW12A
Synchrotron site	Photon Factory
Beamline	AR-NW12A
Temperature [K]	80
Detector technology	CCD
Collection date	2006-06-19
Detector	ADSC QUANTUM 210
Wavelength(s)	1.000
Spacegroup name	P 65 2 2
Unit cell lengths	105.595, 105.595, 142.498
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	43.538 - 2.350
R-factor	0.1997
Rwork	0.199
R-free	0.22280
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4mm1
RMSD bond length	0.002
RMSD bond angle	0.522
Data reduction software	XDS
Data scaling software	XDS
Phasing software	MOLREP
Refinement software	PHENIX ((1.12_2829: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	47.500	2.430
High resolution limit [Å]	2.350	2.350
Number of reflections	20203	1945
<I/σ(I)>	8.3	2.9
Completeness [%]	100.0	100
Redundancy	10.6	10.9
CC(1/2)	0.988	0.942

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	4.8	293	0.1 M sodium acetate pH 4.8 10% (w/v) polyethylene glycol 4000 10 mM rac-G1P 100 mM cesium chloride