6JNM
REF6 ZnF2-4-NAC004-mC3 complex
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL19U1 |
| Synchrotron site | SSRF |
| Beamline | BL19U1 |
| Temperature [K] | 293 |
| Detector technology | PIXEL |
| Collection date | 2017-06-17 |
| Detector | DECTRIS PILATUS3 S 6M |
| Wavelength(s) | 0.97853 |
| Spacegroup name | P 43 |
| Unit cell lengths | 88.516, 88.516, 72.330 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 30.000 - 2.050 |
| R-factor | 0.201 |
| Rwork | 0.200 |
| R-free | 0.22800 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 6jnl |
| RMSD bond length | 0.024 |
| RMSD bond angle | 2.092 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0158) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 2.120 |
| High resolution limit [Å] | 2.050 | 2.050 |
| Rmerge | 0.102 | 1.020 |
| Number of reflections | 35297 | 3546 |
| <I/σ(I)> | 4.241 | |
| Completeness [%] | 100.0 | |
| Redundancy | 12.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 293 | 16% PEG 3,350, 0.03 M citrate acid, 0.07 M Bis-Tris propane pH 7.6 |
