6JNI
Crystal structure of the transcriptional regulator CadR from P. putida in complex with Zinc(II) and DNA
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL17U |
| Synchrotron site | SSRF |
| Beamline | BL17U |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2013-05-11 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 1.0 |
| Spacegroup name | P 1 |
| Unit cell lengths | 71.090, 71.329, 98.797 |
| Unit cell angles | 81.03, 83.52, 73.85 |
Refinement procedure
| Resolution | 30.000 - 2.900 |
| R-factor | 0.21375 |
| Rwork | 0.212 |
| R-free | 0.25443 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 6jgv |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.491 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0135) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 40.000 | 3.000 |
| High resolution limit [Å] | 2.900 | 2.900 |
| Rmerge | 0.510 | |
| Number of reflections | 38729 | 3879 |
| <I/σ(I)> | 7.3 | 2.7 |
| Completeness [%] | 96.9 | 97.2 |
| Redundancy | 2.2 | 2.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 289 | MPEG 2000, magnesium chloride, potassium bromide |
