6JMV
Crystal structure of the GluK3 ligand binding domain complex with SYM and zinc
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID29 |
| Synchrotron site | ESRF |
| Beamline | ID29 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2018-02-11 |
| Detector | DECTRIS PILATUS3 6M |
| Wavelength(s) | 0.972420 |
| Spacegroup name | P 2 21 2 |
| Unit cell lengths | 56.460, 88.030, 130.470 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 72.973 - 1.832 |
| R-factor | 0.2052 |
| Rwork | 0.203 |
| R-free | 0.24240 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3u93 |
| RMSD bond length | 0.010 |
| RMSD bond angle | 0.997 |
| Data reduction software | MOSFLM |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | PHENIX ((1.14_3260: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 88.030 | 1.870 |
| High resolution limit [Å] | 1.830 | 1.830 |
| Rmerge | 0.095 | 0.509 |
| Rmeas | 0.120 | 0.720 |
| Rpim | 0.072 | 0.735 |
| Number of reflections | 57772 | 3540 |
| <I/σ(I)> | 7.9 | 1.3 |
| Completeness [%] | 99.7 | 99.9 |
| Redundancy | 4.7 | 4.9 |
| CC(1/2) | 0.995 | 0.165 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 295 | 50mM HEPES, pH 7.0, 4% PEG 8000, 100mM NaCl |
