6JM7
Crystal structure of Ostrinia furnacalis Group IV chitinase
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NFPSS BEAMLINE BL18U |
| Synchrotron site | NFPSS |
| Beamline | BL18U |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2018-10-14 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.97778 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 66.734, 71.593, 72.452 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 35.797 - 1.572 |
| R-factor | 0.1592 |
| Rwork | 0.159 |
| R-free | 0.17070 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5wup |
| RMSD bond length | 0.007 |
| RMSD bond angle | 0.891 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | PHENIX ((1.11.1_2575: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.600 |
| High resolution limit [Å] | 1.570 | 1.570 |
| Number of reflections | 56099 | 2729 |
| <I/σ(I)> | 10.3 | 5.45 |
| Completeness [%] | 99.8 | 99.5 |
| Redundancy | 9.8 | 8.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 4.2 | 277 | 1600 mM sodium phosphate monobasic, 400 mM potassium phosphate dibasic, 100 mM sodium phosphate dibasic, 100 mM citric acid, pH 4.2 |
