6JLH

Structure of SCGN in complex with a Snap25 peptide

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL19U1
Synchrotron site	SSRF
Beamline	BL19U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2016-11-01
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	0.97921
Spacegroup name	P 21 21 2
Unit cell lengths	104.524, 107.049, 54.770
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	46.960 - 2.370
R-factor	0.2034
Rwork	0.200
R-free	0.23720
RMSD bond length	0.009
RMSD bond angle	1.591
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	AutoSol
Refinement software	REFMAC (5.8.0238)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	2.410
High resolution limit [Å]	2.370	6.430	2.370
Rmerge	0.194	0.167
Rmeas	0.197	0.170
Rpim	0.035	0.029	0.342
Total number of observations	964927
Number of reflections	25808	1444	1167
<I/σ(I)>	4.6
Completeness [%]	99.6	100	91.8
Redundancy	37.4	34.9	20.9
CC(1/2)		0.991	0.639

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293	0.1 M glycocine, 0.1 M Tris Bicine pH 8.5, 12.5% MPD, 12.5% PEG 1000, 12.5% PEG 3350