6JLF

Crystal structure of a bacterial Bestrophin homolog from Klebsiella pneumoniae D179A mutation - HR

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-C
Synchrotron site	APS
Beamline	24-ID-C
Temperature [K]	100
Detector technology	PIXEL
Collection date	2018-12-11
Detector	DECTRIS PILATUS 6M-F
Wavelength(s)	0.9792
Spacegroup name	P 21 21 21
Unit cell lengths	113.970, 159.468, 161.901
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	93.195 - 2.548
R-factor	0.2307
Rwork	0.230
R-free	0.27590
RMSD bond length	0.011
RMSD bond angle	1.213
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHASER
Refinement software	PHENIX ((1.11.1_2575: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	93.195	2.640
High resolution limit [Å]	2.548	2.548
Number of reflections	95113
<I/σ(I)>	18.74
Completeness [%]	98.2
Redundancy	3.4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293	0.05M ZnAce, 6.6% PEG 8000, 0.1M Na-Cacodylat (pH 6.0), 6% EG