6JL7

crystal structure of TBC1D23 N terminal domain

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL19U1
Synchrotron site	SSRF
Beamline	BL19U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2018-10-01
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	0.9785
Spacegroup name	P 65
Unit cell lengths	151.736, 151.736, 38.604
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	49.720 - 2.500
R-factor	0.2191
Rwork	0.217
R-free	0.25190
Structure solution method	SAD
RMSD bond length	0.003
RMSD bond angle	0.742
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	AutoSol
Refinement software	REFMAC (5.8.0222)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	2.540
High resolution limit [Å]	2.500	6.780	2.500
Rmerge	0.158	0.111
Rmeas	0.159	0.115
Rpim	0.051	0.031	0.537
Number of reflections	17980	972	903
<I/σ(I)>	3.5
Completeness [%]	100.0	100	100
Redundancy	14.1	13.6	14
CC(1/2)		0.994	0.453

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293	0.2 M MES pH6.5, 0.5 M Ammonium nitrate, 25% polyethylene glycol 400