6JL1

Crystal structure of VvPlpA G389D from Vibrio vulnificus

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL19U1
Synchrotron site	SSRF
Beamline	BL19U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2018-11-18
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	0.9791
Spacegroup name	P 31 2 1
Unit cell lengths	54.397, 54.397, 233.181
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	47.100 - 2.290
R-factor	0.195
Rwork	0.193
R-free	0.23500
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6jkz
RMSD bond length	0.010
RMSD bond angle	1.020
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	BUSTER (2.10.2)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	47.103	2.327
High resolution limit [Å]	2.288	2.288
Rmeas	0.097	0.734
Rpim	0.023	0.180
Number of reflections	19033	917
<I/σ(I)>	17.3	3
Completeness [%]	100.0	100
Redundancy	18.6	16.3
CC(1/2)	0.999	0.969

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	5 mg/ml in 10 mM HEPES, 150 mM NaCl, pH 7.5 was mixed with equal volume of reservoir solution (200 mM potassium sodium tartrate tetrahydrate, 180 mM NDSB-201, 8% polyethylene glycol 1500, and 20% glycerol)