6JKZ
Crystal structure of VvPlpA from Vibrio vulnificus
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRF BEAMLINE BL17U1 |
Synchrotron site | SSRF |
Beamline | BL17U1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2018-04-15 |
Detector | DECTRIS EIGER X 16M |
Wavelength(s) | 0.9792 |
Spacegroup name | P 31 2 1 |
Unit cell lengths | 54.158, 54.158, 233.143 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 45.981 - 1.397 |
R-factor | 0.1666 |
Rwork | 0.166 |
R-free | 0.18740 |
Structure solution method | SAD |
RMSD bond length | 0.008 |
RMSD bond angle | 0.951 |
Data reduction software | XDS |
Data scaling software | Aimless |
Phasing software | SHELXDE |
Refinement software | PHENIX ((1.10.1_2155: ???)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 46.902 | 1.421 |
High resolution limit [Å] | 1.397 | 1.397 |
Rmeas | 0.069 | 0.926 |
Rpim | 0.016 | 0.255 |
Number of reflections | 79933 | 3826 |
<I/σ(I)> | 22 | |
Completeness [%] | 99.7 | 96.6 |
Redundancy | 18.8 | 12.8 |
CC(1/2) | 0.998 | 0.768 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 293 | 1.5 ul of protein solution (8 mg/ml in the buffer 10 mM HEPES, 150 mM NaCl, pH 7.5) and 1.5 ul of reservoir solution containing 500 mM NaCl, 10 mM MgCl2, 180 mM NDSB-201, 30% glycerine. |