6JKJ
Crystal structure of human SPSB2 in the apo-state
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL17U |
| Synchrotron site | SSRF |
| Beamline | BL17U |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2018-06-22 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 0.9792 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 32.450, 75.100, 79.200 |
| Unit cell angles | 90.00, 101.38, 90.00 |
Refinement procedure
| Resolution | 34.490 - 1.900 |
| R-factor | 0.21935 |
| Rwork | 0.217 |
| R-free | 0.26986 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3emw |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.555 |
| Data reduction software | xia2 |
| Data scaling software | Aimless |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0238) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 34.490 | 1.950 |
| High resolution limit [Å] | 1.900 | 1.900 |
| Rmerge | 0.154 | 0.668 |
| Number of reflections | 28566 | 2029 |
| <I/σ(I)> | 7.6 | |
| Completeness [%] | 97.3 | |
| Redundancy | 3.4 | 3.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 289 | 0.2M Ammonium acetate, 0.1M BIS-TRIS pH 5.5, 25% w/v Polyethylene glycol 3350 |
