6JKH
The NAD+-bound form of human NSDHL
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL44XU |
| Synchrotron site | SPring-8 |
| Beamline | BL44XU |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2016-04-20 |
| Detector | MARMOSAIC 300 mm CCD |
| Wavelength(s) | 0.9 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 50.027, 77.366, 53.993 |
| Unit cell angles | 90.00, 96.80, 90.00 |
Refinement procedure
| Resolution | 27.410 - 3.000 |
| R-factor | 0.17632 |
| Rwork | 0.173 |
| R-free | 0.23841 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3wj7 |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.490 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0238) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 3.050 |
| High resolution limit [Å] | 3.000 | 3.000 |
| Rmerge | 0.146 | 0.865 |
| Number of reflections | 8240 | 410 |
| <I/σ(I)> | 22.2 | |
| Completeness [%] | 99.8 | |
| Redundancy | 3.98 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 293 | 0.2 M calcium chloride, 44% PEG400, 0.1 M Tris, and pH 8.0 |
