6JKG
The NAD+-free form of human NSDHL
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PAL/PLS BEAMLINE 11C |
| Synchrotron site | PAL/PLS |
| Beamline | 11C |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2018-07-31 |
| Detector | DECTRIS PILATUS3 S 6M |
| Wavelength(s) | 0.9794 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 49.481, 58.534, 75.601 |
| Unit cell angles | 90.00, 108.34, 90.00 |
Refinement procedure
| Resolution | 29.801 - 2.900 |
| R-factor | 0.231 |
| Rwork | 0.228 |
| R-free | 0.29070 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 6jkh |
| RMSD bond length | 0.006 |
| RMSD bond angle | 0.907 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | MOLREP |
| Refinement software | PHENIX ((1.14_3260: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 2.900 |
| High resolution limit [Å] | 2.900 | 2.850 |
| Rmerge | 0.084 | 0.287 |
| Number of reflections | 9118 | 425 |
| <I/σ(I)> | 22.2 | |
| Completeness [%] | 98.5 | |
| Redundancy | 3.29 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 293 | 0.5 M magnesium chloride, 0.02 M lithium chloride, 33% PEG1000, 0.02 M Glycine, and pH 10.0 |
