6JJM

Crystal structure of Mumps virus hemagglutinin-neuraminidase bound to the oligosaccharide portion of the GM2 ganglioside

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SPRING-8 BEAMLINE BL26B2
Synchrotron site	SPring-8
Beamline	BL26B2
Temperature [K]	100
Detector technology	CCD
Collection date	2017-07-15
Detector	RAYONIX MX225-HS
Wavelength(s)	1
Spacegroup name	P 61
Unit cell lengths	137.257, 137.257, 178.009
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	44.928 - 2.049
R-factor	0.1839
Rwork	0.183
R-free	0.20100
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.003
RMSD bond angle	0.796
Phasing software	PHASER
Refinement software	PHENIX ((1.13_2998: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	44.940	2.090
High resolution limit [Å]	2.049	2.050
Number of reflections	118595
<I/σ(I)>	29.4
Completeness [%]	99.8
Redundancy	21.1

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION		293	sodium acetate, ammonium sulfate, glycerol