6JJ7
Crystal structure of OsHXK6-Glc complex
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL17U1 |
| Synchrotron site | SSRF |
| Beamline | BL17U1 |
| Temperature [K] | 95 |
| Detector technology | CCD |
| Collection date | 2018-06-07 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.987 |
| Spacegroup name | P 32 2 1 |
| Unit cell lengths | 131.569, 131.569, 188.782 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 45.470 - 2.900 |
| R-factor | 0.2019 |
| Rwork | 0.200 |
| R-free | 0.24800 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4qs7 |
| RMSD bond length | 0.014 |
| RMSD bond angle | 1.460 |
| Data reduction software | MOSFLM |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | PHENIX ((1.10.1_2155: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 45.470 | 3.004 |
| High resolution limit [Å] | 2.900 | 2.900 |
| Rmerge | 0.031 | 0.145 |
| Number of reflections | 42411 | 4180 |
| <I/σ(I)> | 15.15 | 4.54 |
| Completeness [%] | 100.0 | |
| Redundancy | 2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 277.15 | 0.2 M lithium sulfate, 0.1 M ADA 6.5, 30% v/v PEG 400, and the additive 29 (C5) from kit Additive ScreenTM HR2-428 (Hampton Research),2mM glucose |
