6JIX

The cyrstal structure of taurine:2-oxoglutarate aminotransferase from Bifidobacterium kashiwanohense, in complex with PLP and glutamate

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL18U1
Synchrotron site	SSRF
Beamline	BL18U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2019-01-13
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	0.9792
Spacegroup name	P 1 21 1
Unit cell lengths	68.390, 164.544, 109.240
Unit cell angles	90.00, 105.81, 90.00

Refinement procedure

Resolution	44.289 - 2.647
R-factor	0.1687
Rwork	0.167
R-free	0.22690
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5lh9
Data reduction software	HKL-3000
Data scaling software	HKL-3000
Phasing software	PHENIX
Refinement software	PHENIX (1.14_3247)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	7.190	2.700
High resolution limit [Å]	2.640	5.710	2.650
Rmerge	0.112	0.067	0.490
Rmeas	0.133	0.079	0.578
Rpim	0.071	0.042	0.304
Number of reflections	66599	3354	3302
<I/σ(I)>	4
Completeness [%]	98.9	99.4	98.5
Redundancy	3.4	3.5	3.5
CC(1/2)		0.994	0.837

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	5	295	0.1M MES, pH 5.0, 0.2M ammonium chloride, 10% W/V PEG 6000, 0.5mM PLP, 100mM glutamate