6JIL
Crystal structure of D-cycloserine synthetase DcsG
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL38B1 |
| Synchrotron site | SPring-8 |
| Beamline | BL38B1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2015-01-20 |
| Detector | RAYONIX MX-225 |
| Wavelength(s) | 0.97864 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 56.450, 120.730, 102.810 |
| Unit cell angles | 90.00, 101.01, 90.00 |
Refinement procedure
| Resolution | 29.760 - 2.320 |
| R-factor | 0.195 |
| Rwork | 0.195 |
| R-free | 0.25100 |
| Structure solution method | SAD |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.300 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | SHARP |
| Refinement software | CNS (1.2) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 100.000 | 2.400 |
| High resolution limit [Å] | 2.320 | 2.320 |
| Rmerge | 0.117 | 0.447 |
| Number of reflections | 55347 | |
| <I/σ(I)> | 10.1 | 1.8 |
| Completeness [%] | 95.3 | 82.4 |
| Redundancy | 3.6 | 2.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 298 | 1.0 M potassium sodium L-tartrate, 0.1 M Tris-HCl buffer (pH 7.5) |
