6JIK

Aspergillus fumigatus Rho1 GsGTP

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SEALED TUBE
Source details	RIGAKU MICROMAX-003
Temperature [K]	100
Detector technology	CCD
Collection date	2019-01-08
Detector	RIGAKU SATURN 944
Wavelength(s)	1.5418
Spacegroup name	P 21 21 21
Unit cell lengths	37.250, 65.700, 75.010
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	30.110 - 2.350
R-factor	0.23448
Rwork	0.232
R-free	0.27505
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5zvp
RMSD bond length	0.002
RMSD bond angle	1.214
Data reduction software	MOSFLM
Data scaling software	Aimless
Phasing software	MOLREP
Refinement software	REFMAC (5.8.0238)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	30.110	2.430
High resolution limit [Å]	2.350	2.350
Rmerge	0.192	0.370
Rpim	0.115	0.246
Number of reflections	8136	767
<I/σ(I)>	5.8	3.2
Completeness [%]	99.9	99.4
Redundancy	6.5	5.3
CC(1/2)	0.982	0.909

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293.15	crystals were obtained in Morpheus commercial crystallization screen, Imidazole and MES monohydrate (acid) at pH 6.5, 40% v/v PEG 500 MME and 20 % w/v PEG 20000, and 0.2M 1,6-Hexanediol, 0.2M 1-Butanol 0.2M 1,2-Propanediol, 0.2M 2-Propanol, 0.2M 1,4- Butanediol and 0.2M 1,3-Propanediol.