6JHO

The complex crystal structure of Cagbeta with CagZ revealed a novel regulatory mechanism for T4SS coupling ATPase in Helicobacter pylori

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	AichiSR BEAMLINE BL2S1
Synchrotron site	AichiSR
Beamline	BL2S1
Temperature [K]	100
Detector technology	CCD
Collection date	2018-07-15
Detector	ADSC QUANTUM 315
Wavelength(s)	0.97775
Spacegroup name	P 41 21 2
Unit cell lengths	96.298, 96.298, 212.658
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	48.149 - 2.100
R-factor	0.1923
Rwork	0.191
R-free	0.22110
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1s2x
RMSD bond length	0.008
RMSD bond angle	0.826
Data reduction software	HKL-3000
Data scaling software	SCALEPACK
Phasing software	PHENIX
Refinement software	PHENIX (1.11.1_2575)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	2.030
High resolution limit [Å]	2.000	5.430	2.000
Rmerge	0.083	0.034	1.562
Rmeas	0.087	0.036	1.639
Rpim	0.024	0.010	0.488
Number of reflections	68411	3768	3339
<I/σ(I)>	4
Completeness [%]	99.9	100	99
Redundancy	12.3	12.2	10.3
CC(1/2)		0.999	0.543

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		289	CAPSO, PEG8k, sodium chloride