6JHJ
Structure of Marine bacterial laminarinase mutant-E135A
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PHOTON FACTORY BEAMLINE BL-5A |
| Synchrotron site | Photon Factory |
| Beamline | BL-5A |
| Temperature [K] | 95 |
| Detector technology | PIXEL |
| Collection date | 2018-11-17 |
| Detector | DECTRIS PILATUS3 2M |
| Wavelength(s) | 1.0000 |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 66.808, 94.606, 72.442 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 39.607 - 1.690 |
| R-factor | 0.15191 |
| Rwork | 0.150 |
| R-free | 0.18483 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4crq |
| RMSD bond length | 0.019 |
| RMSD bond angle | 1.725 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHENIX |
| Refinement software | REFMAC (5.8.0135) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 47.300 | 1.790 |
| High resolution limit [Å] | 1.690 | 1.690 |
| Rmerge | 0.044 | 0.593 |
| Number of reflections | 22574 | 945 |
| <I/σ(I)> | 24.2 | |
| Completeness [%] | 96.5 | 73.9 |
| Redundancy | 6 | 3.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 293 | 5% (v/v) ethonal, 28% PEG20000 100 mM MES pH7.0 |
