6JH5
Structure of Marine bacterial laminarinase
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PHOTON FACTORY BEAMLINE BL-5A |
| Synchrotron site | Photon Factory |
| Beamline | BL-5A |
| Temperature [K] | 95 |
| Detector technology | PIXEL |
| Collection date | 2018-11-17 |
| Detector | DECTRIS PILATUS3 X 2M |
| Wavelength(s) | 1.000 |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 66.775, 101.879, 75.529 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 55.850 - 1.540 |
| R-factor | 0.15091 |
| Rwork | 0.150 |
| R-free | 0.17462 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4crq |
| RMSD bond length | 0.021 |
| RMSD bond angle | 1.824 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHENIX |
| Refinement software | REFMAC (5.8.0135) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 55.850 | 1.560 |
| High resolution limit [Å] | 1.540 | 1.540 |
| Rmerge | 0.032 | 0.266 |
| Number of reflections | 34923 | 739 |
| <I/σ(I)> | 32.3 | 2.8 |
| Completeness [%] | 90.4 | 39.2 |
| Redundancy | 5.9 | 2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 293 | 15% (v/v) ethonal, 100 mM MES pH7.0 |
