6JGV
Crystal structure of the transcriptional regulator CadR from P. putida
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL17U |
| Synchrotron site | SSRF |
| Beamline | BL17U |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2012-11-08 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 1.0 |
| Spacegroup name | P 31 |
| Unit cell lengths | 47.304, 47.304, 100.385 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 30.000 - 2.200 |
| R-factor | 0.2099 |
| Rwork | 0.208 |
| R-free | 0.24983 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 6jgf |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.243 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0103) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 2.280 |
| High resolution limit [Å] | 2.200 | 2.200 |
| Rmerge | 0.307 | |
| Number of reflections | 12450 | 1228 |
| <I/σ(I)> | 21.1 | 3.7 |
| Completeness [%] | 97.5 | 95.3 |
| Redundancy | 5.9 | 4.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8.5 | 289 | PEG 3350, magnesum chloride, Tris-HCl |
