6JFV

The crystal structure of 2B-2B complex from keratins 5 and 14 (C367A mutant of K14)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 23-ID-B
Synchrotron site	APS
Beamline	23-ID-B
Temperature [K]	100
Detector technology	CCD
Collection date	2011-11-11
Detector	MAR CCD 165 mm
Wavelength(s)	0.97838
Spacegroup name	P 41
Unit cell lengths	56.149, 56.149, 222.054
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	37.380 - 2.600
R-factor	0.203
Rwork	0.201
R-free	0.24580
RMSD bond length	0.006
RMSD bond angle	0.759
Data reduction software	HKL-2000
Data scaling software	SCALEPACK
Phasing software	PHENIX
Refinement software	PHENIX ((phenix.refine))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	37.380	2.690
High resolution limit [Å]	2.600	2.600
Number of reflections	20216
<I/σ(I)>	8.91
Completeness [%]	96.1
Redundancy	4.6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		300	12%(w/v) PEG-8000, 0.1M sodium cacodylate pH 6.5, and 0.2 M calcium acetate hydrate