6JFL

Nucleotide-free Mitofusin2 (MFN2)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL17U1
Synchrotron site	SSRF
Beamline	BL17U1
Temperature [K]	100
Detector technology	CCD
Collection date	2017-05-26
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.97915
Spacegroup name	P 1 21 1
Unit cell lengths	84.482, 128.424, 91.329
Unit cell angles	90.00, 106.27, 90.00

Refinement procedure

Resolution	36.337 - 2.806
R-factor	0.2108
Rwork	0.207
R-free	0.28320
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6jfm
RMSD bond length	0.009
RMSD bond angle	1.184
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	MOLREP
Refinement software	PHENIX ((1.10.1_2155: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	48.700	2.980
High resolution limit [Å]	2.800	2.810
Rmeas	0.128	0.586
Number of reflections	44958	6966
<I/σ(I)>	10.42
Completeness [%]	98.0
Redundancy	3.3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293.15	0.19 M Calcium chloride 0.095 M HEPES PH7.5 26.6% (V/V) PEG400 5% (V/V) Glycerol