6JFK
GDP bound Mitofusin2 (MFN2)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL19U1 |
| Synchrotron site | SSRF |
| Beamline | BL19U1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2017-04-02 |
| Detector | DECTRIS PILATUS3 S 6M |
| Wavelength(s) | 0.977760 |
| Spacegroup name | P 41 21 2 |
| Unit cell lengths | 49.624, 49.624, 388.065 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 49.223 - 1.997 |
| R-factor | 0.1782 |
| Rwork | 0.176 |
| R-free | 0.21210 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5gom |
| RMSD bond length | 0.007 |
| RMSD bond angle | 0.903 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | MOLREP |
| Refinement software | PHENIX ((1.10.1_2155: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 49.600 | 2.120 |
| High resolution limit [Å] | 1.997 | 2.000 |
| Rmeas | 0.064 | |
| Rpim | 0.520 | |
| Number of reflections | 34454 | 5321 |
| <I/σ(I)> | 18.31 | |
| Completeness [%] | 99.3 | |
| Redundancy | 6.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 293.15 | 0.2 M Lithium citrate tribasic tetrahydrate, 35% (V/V) Glycerol ethoxylate |
