6JEP
Structure of a neutralizing antibody bound to the Zika envelope protein domain III
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL17U |
| Synchrotron site | SSRF |
| Beamline | BL17U |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2017-05-28 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.97915 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 66.463, 126.984, 70.353 |
| Unit cell angles | 90.00, 96.00, 90.00 |
Refinement procedure
| Resolution | 35.646 - 2.316 |
| R-factor | 0.1689 |
| Rwork | 0.166 |
| R-free | 0.21930 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Phasing software | HKL-3000 |
| Refinement software | PHENIX (1.14_3247) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 35.646 | 2.360 |
| High resolution limit [Å] | 2.316 | 2.320 |
| Number of reflections | 50308 | |
| <I/σ(I)> | 16.15 | |
| Completeness [%] | 99.7 | |
| Redundancy | 3.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 289 | MPD, PEG1500, Sodium acetate/Acetic acid |
