6JDJ
Crystal structure of AcrIIC2 dimer in complex with partial Nme1Cas9
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRF BEAMLINE BL19U1 |
Synchrotron site | SSRF |
Beamline | BL19U1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2018-03-30 |
Detector | DECTRIS PILATUS3 S 6M |
Wavelength(s) | 0.97891 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 61.439, 77.347, 106.885 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 38.673 - 2.600 |
R-factor | 0.1954 |
Rwork | 0.194 |
R-free | 0.22650 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 6jd7 |
RMSD bond length | 0.005 |
RMSD bond angle | 0.996 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHASER |
Refinement software | PHENIX ((1.14_3247: ???)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.640 |
High resolution limit [Å] | 2.600 | 2.600 |
Rmerge | 0.154 | 0.900 |
Rmeas | 0.161 | 0.952 |
Rpim | 0.045 | 0.301 |
Number of reflections | 16424 | 803 |
<I/σ(I)> | 2.3 | 2.3 |
Completeness [%] | 99.9 | 99.4 |
Redundancy | 12.1 | 9.4 |
CC(1/2) | 0.995 | 0.815 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | EVAPORATION | 7 | 289 | 0.1M HEPES 7.9, 14% PEG 20000 |