6JD7
Crystal structure of anti-CRISPR protein AcrIIC2 dimer
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL19U1 |
| Synchrotron site | SSRF |
| Beamline | BL19U1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2017-03-06 |
| Detector | DECTRIS PILATUS3 S 6M |
| Wavelength(s) | 0.979 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 105.559, 73.532, 81.087 |
| Unit cell angles | 90.00, 129.80, 90.00 |
Refinement procedure
| Resolution | 39.992 - 2.450 |
| R-factor | 0.1941 |
| Rwork | 0.194 |
| R-free | 0.19700 |
| Structure solution method | SAD |
| RMSD bond length | 0.020 |
| RMSD bond angle | 1.479 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | PHENIX ((1.14_3247: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.490 |
| High resolution limit [Å] | 2.450 | 2.450 |
| Rmerge | 0.074 | 0.288 |
| Rmeas | 0.083 | 0.321 |
| Rpim | 0.037 | |
| Number of reflections | 17826 | 855 |
| <I/σ(I)> | 19.7 | 4.57 |
| Completeness [%] | 99.7 | 99.7 |
| Redundancy | 5.1 | 5.1 |
| CC(1/2) | 0.988 | 0.959 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | COUNTER-DIFFUSION | 6.5 | 289 | 0.05M Na cacodylate pH 6.5, 0.1M Ammonium acetate, 0.015M Mg acetate, 10% Isopropanol |
