6JCU

Crystal structure of an actin monomer in complex with a nucleator Cordon-Bleu WH2-motif peptide mutant. T22V, H11R

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSRRC BEAMLINE TPS 05A
Synchrotron site	NSRRC
Beamline	TPS 05A
Temperature [K]	100
Detector technology	CCD
Collection date	2017-03-29
Detector	RAYONIX MX300-HS
Wavelength(s)	1.0
Spacegroup name	P 21 21 2
Unit cell lengths	77.946, 184.213, 55.673
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	19.936 - 2.297
R-factor	0.1825
Rwork	0.180
R-free	0.22980
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5ypu
RMSD bond length	0.009
RMSD bond angle	1.296
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX ((1.13_2998: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	92.106	2.364
High resolution limit [Å]	2.297	2.297
Number of reflections	835894
<I/σ(I)>	14.6
Completeness [%]	98.2
Redundancy	6.7

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		298.15	0.2M Ammonium nitrate, 20% PEG 3350