6JCO
Crystal structure of calcium free human gelsolin amyloid mutant D187N
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | DIAMOND BEAMLINE I02 |
Synchrotron site | Diamond |
Beamline | I02 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2014-11-17 |
Detector | DECTRIS PILATUS3 6M |
Wavelength(s) | 0.979 |
Spacegroup name | P 4 21 2 |
Unit cell lengths | 170.130, 170.130, 151.480 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 69.193 - 2.880 |
R-factor | 0.2088 |
Rwork | 0.206 |
R-free | 0.26730 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3ffn |
RMSD bond length | 0.010 |
RMSD bond angle | 1.109 |
Data reduction software | xia2 |
Data scaling software | XSCALE |
Phasing software | PHASER |
Refinement software | PHENIX ((1.13_2998)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 69.193 | 2.980 |
High resolution limit [Å] | 2.880 | 2.880 |
Number of reflections | 50758 | |
<I/σ(I)> | 3.51 | |
Completeness [%] | 99.9 | |
Redundancy | 12.6 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION | 8.5 | 298.1 | 15% glycerol, 1.5M ammonium sulfate, 0.1M tris HCl pH8.5 |