6JBU

High resolution crystal structure of human FLRT3 LRR domain in complex with mouse CIRL3 Olfactomedin like domain

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL17U1
Synchrotron site	SSRF
Beamline	BL17U1
Temperature [K]	100
Detector technology	CCD
Collection date	2015-05-06
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.97912
Spacegroup name	P 31 2 1
Unit cell lengths	121.135, 121.135, 83.413
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	44.403 - 1.849
R-factor	0.209
Rwork	0.208
R-free	0.23430
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4rmk 4yeb 5cmn
RMSD bond length	0.010
RMSD bond angle	1.115
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX ((1.14_3260: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	1.880
High resolution limit [Å]	1.849	1.849
Rmerge	0.078	0.884
Rmeas	0.084	0.955
Rpim	0.032	0.357
Number of reflections	60397	2986
<I/σ(I)>	28.133	2.737
Completeness [%]	99.7	100
Redundancy	6.9	6.9
CC(1/2)	0.999	0.822

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	8.5	293	560 mM sodium monobasic phosphate/potassium dibasic phosphate, 0.1 M Tris pH 8.5, 1.5% MPD