6JBP
Structure of MP-4 from Mucuna pruriens at 2.22 Angstroms
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SEALED TUBE |
| Source details | Xenocs GeniX 3D Cu HF |
| Temperature [K] | 298 |
| Detector technology | IMAGE PLATE |
| Collection date | 2017-11-01 |
| Detector | MARRESEARCH |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 31 2 1 |
| Unit cell lengths | 75.460, 75.460, 94.230 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 38.218 - 2.217 |
| R-factor | 0.1814 |
| Rwork | 0.179 |
| R-free | 0.22030 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5dss |
| RMSD bond length | 0.006 |
| RMSD bond angle | 0.773 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | PHENIX ((1.14_3260: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 53.700 | 2.330 |
| High resolution limit [Å] | 2.210 | 2.210 |
| Rmerge | 0.067 | 0.322 |
| Rmeas | 0.072 | 0.348 |
| Rpim | 0.026 | 0.129 |
| Number of reflections | 15843 | 2151 |
| <I/σ(I)> | 23.8 | 5.9 |
| Completeness [%] | 99.2 | 94.2 |
| Redundancy | 7.3 | 7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 9.5 | 298 | 50mM Tris-HCl pH 9.5, 1.5M ammonium sulfate |
