6JBA
Crystal structure of ABC transporter alpha-glycoside-binding mutant protein W287A in complex with palatinose
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU MICROMAX-007 HF |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2018-04-22 |
| Detector | RIGAKU RAXIS IV++ |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 41 21 2 |
| Unit cell lengths | 84.100, 84.100, 146.020 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 72.880 - 2.000 |
| R-factor | 0.1525 |
| Rwork | 0.151 |
| R-free | 0.18630 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 6j9w |
| RMSD bond length | 0.017 |
| RMSD bond angle | 1.787 |
| Data reduction software | iMOSFLM (7.2.2) |
| Data scaling software | Aimless (0.7.3) |
| Phasing software | PHASER (2.8.2) |
| Refinement software | REFMAC (5.8.0238) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 72.880 | 8.940 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Rmerge | 0.140 | 0.574 |
| Rmeas | 0.158 | 0.653 |
| Rpim | 0.073 | 0.308 |
| Number of reflections | 36237 | 2647 |
| <I/σ(I)> | 10.4 | 3.4 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 8.5 | 8.4 |
| CC(1/2) | 0.990 | 0.917 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | MICROBATCH | 6.4 | 277 | 0.04 M Citric Acid, 0.06 M Bis-Tris Propane, 16% PEG 3350 |
