6JB9

Crystal structure of nanobody D3-L11 (unbound form)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PHOTON FACTORY BEAMLINE BL-5A
Synchrotron site	Photon Factory
Beamline	BL-5A
Temperature [K]	100
Detector technology	CCD
Collection date	2015-11-16
Detector	ADSC QUANTUM 315r
Wavelength(s)	1.0000
Spacegroup name	P 1 21 1
Unit cell lengths	26.190, 53.360, 36.210
Unit cell angles	90.00, 91.52, 90.00

Refinement procedure

Resolution	26.690 - 1.150
R-factor	0.10211
Rwork	0.102
R-free	0.12903
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6jb2
RMSD bond length	0.021
RMSD bond angle	2.167
Data reduction software	MOSFLM (7.1.0)
Data scaling software	SCALA (3.3.21)
Phasing software	PHASER (2.5.6)
Refinement software	REFMAC (5.8.0238)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	26.700	1.210
High resolution limit [Å]	1.150	1.150
Rmerge	0.070	0.210
Number of reflections	34665	4414
<I/σ(I)>	14.1	6
Completeness [%]	97.9	85.9
Redundancy	5.2
CC(1/2)	0.997	0.952

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7	293.15	100mM TRIS HCl, 100mM NaCl, 2.0M Ammonium sulfate