6JB5
Crystal structure of nanobody D3-L11 mutant Y102A in complex with hen egg-white lysozyme (form II)
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | PHOTON FACTORY BEAMLINE AR-NW12A |
Synchrotron site | Photon Factory |
Beamline | AR-NW12A |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2015-05-18 |
Detector | ADSC QUANTUM 270 |
Wavelength(s) | 1.0000 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 44.010, 49.440, 57.940 |
Unit cell angles | 90.00, 93.79, 90.00 |
Refinement procedure
Resolution | 49.440 - 1.550 |
R-factor | 0.13297 |
Rwork | 0.132 |
R-free | 0.16856 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 6jb2 |
RMSD bond length | 0.014 |
RMSD bond angle | 1.577 |
Data reduction software | MOSFLM (7.1.0) |
Data scaling software | SCALA (3.3.21) |
Phasing software | PHASER (2.5.6) |
Refinement software | REFMAC (5.8.0189) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 49.440 | 1.630 |
High resolution limit [Å] | 1.550 | 1.550 |
Rmerge | 0.094 | 0.594 |
Number of reflections | 36086 | 5168 |
<I/σ(I)> | 10.8 | 2.4 |
Completeness [%] | 99.7 | 98 |
Redundancy | 5.1 | 4.3 |
CC(1/2) | 0.996 | 0.717 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 8 | 293.15 | 20mM Tris-HCl pH 8.0, 100mM NaCl, 100mM LiCl, 18 % PEG 3350 |