6JB5
Crystal structure of nanobody D3-L11 mutant Y102A in complex with hen egg-white lysozyme (form II)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PHOTON FACTORY BEAMLINE AR-NW12A |
| Synchrotron site | Photon Factory |
| Beamline | AR-NW12A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2015-05-18 |
| Detector | ADSC QUANTUM 270 |
| Wavelength(s) | 1.0000 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 44.010, 49.440, 57.940 |
| Unit cell angles | 90.00, 93.79, 90.00 |
Refinement procedure
| Resolution | 49.440 - 1.550 |
| R-factor | 0.13297 |
| Rwork | 0.132 |
| R-free | 0.16856 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 6jb2 |
| RMSD bond length | 0.014 |
| RMSD bond angle | 1.577 |
| Data reduction software | MOSFLM (7.1.0) |
| Data scaling software | SCALA (3.3.21) |
| Phasing software | PHASER (2.5.6) |
| Refinement software | REFMAC (5.8.0189) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 49.440 | 1.630 |
| High resolution limit [Å] | 1.550 | 1.550 |
| Rmerge | 0.094 | 0.594 |
| Number of reflections | 36086 | 5168 |
| <I/σ(I)> | 10.8 | 2.4 |
| Completeness [%] | 99.7 | 98 |
| Redundancy | 5.1 | 4.3 |
| CC(1/2) | 0.996 | 0.717 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8 | 293.15 | 20mM Tris-HCl pH 8.0, 100mM NaCl, 100mM LiCl, 18 % PEG 3350 |
