6JAK
OtsA apo structure
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL17U1 |
| Synchrotron site | SSRF |
| Beamline | BL17U1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2018-05-27 |
| Detector | MARRESEARCH |
| Wavelength(s) | 0.9792 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 87.470, 119.660, 99.430 |
| Unit cell angles | 90.00, 91.88, 90.00 |
Refinement procedure
| Resolution | 29.687 - 2.410 |
| R-factor | 0.1828 |
| Rwork | 0.180 |
| R-free | 0.23140 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1uqu |
| RMSD bond length | 0.014 |
| RMSD bond angle | 1.493 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHASER |
| Refinement software | PHENIX ((1.10.1_2155: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 87.420 | 2.470 |
| High resolution limit [Å] | 2.410 | 2.410 |
| Rmerge | 0.134 | 0.800 |
| Rpim | 0.080 | 0.521 |
| Number of reflections | 75262 | 7695 |
| <I/σ(I)> | 8.2 | 2.1 |
| Completeness [%] | 95.3 | |
| Redundancy | 7.2 | |
| CC(1/2) | 0.993 | 0.685 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8.5 | 291 | 0.1M Tris pH 8.5, 25% PEG 3350 |
