6J9V
Crystal structure of Trypanosoma brucei gambiense glycerol kinase complex with ADP.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL44XU |
| Synchrotron site | SPring-8 |
| Beamline | BL44XU |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2018-12-14 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 0.90000 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 62.416, 120.948, 154.097 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 20.000 - 2.400 |
| R-factor | 0.19653 |
| Rwork | 0.193 |
| R-free | 0.25416 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3wxl |
| RMSD bond length | 0.014 |
| RMSD bond angle | 1.763 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0103) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.550 |
| High resolution limit [Å] | 2.400 | 2.400 |
| Rmerge | 0.054 | 0.654 |
| Number of reflections | 46371 | 14037 |
| <I/σ(I)> | 15.71 | 2.1 |
| Completeness [%] | 99.8 | 99 |
| Redundancy | 5.3 | 5.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 293 | 8-18% PEG 400, 0.1M HEPES, 11% 1,6-HEXANEDIOL |
