6J9Q

Crystal structure of Trypanosoma brucei gambiense glycerol kinase complex with AMP-PNP.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PHOTON FACTORY BEAMLINE BL-17A
Synchrotron site	Photon Factory
Beamline	BL-17A
Temperature [K]	100
Detector technology	PIXEL
Collection date	2018-11-30
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.98000
Spacegroup name	P 1 21 1
Unit cell lengths	62.598, 123.149, 154.223
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	19.880 - 2.700
R-factor	0.19492
Rwork	0.191
R-free	0.26934
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3wxl
RMSD bond length	0.009
RMSD bond angle	1.445
Data reduction software	XDS
Data scaling software	XDS
Phasing software	MOLREP
Refinement software	REFMAC (5.8.0103)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.860
High resolution limit [Å]	2.700	2.700
Rmerge	0.054	0.448
Rpim	0.068
Number of reflections	62097	20049
<I/σ(I)>	12.5	2
Completeness [%]	98.4	98
Redundancy	2.7	2.7
CC(1/2)	0.998

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.5	293	8-18% PEG 400, 0.1M HEPES, 11% 1,6-HEXANEDIOL