6J9M
NmeBH+AcrIIC2
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL18U1 |
| Synchrotron site | SSRF |
| Beamline | BL18U1 |
| Temperature [K] | 193 |
| Detector technology | PIXEL |
| Collection date | 2017-12-04 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.9785 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 46.385, 81.996, 76.063 |
| Unit cell angles | 90.00, 96.44, 90.00 |
Refinement procedure
| Resolution | 41.475 - 2.394 |
| R-factor | 0.1817 |
| Rwork | 0.179 |
| R-free | 0.22230 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 6j9k |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.185 |
| Data scaling software | HKL-3000 |
| Phasing software | PHENIX |
| Refinement software | PHENIX (1.8.4_1496) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.430 |
| High resolution limit [Å] | 2.390 | 2.390 |
| Rmerge | 0.097 | 0.274 |
| Number of reflections | 22256 | 1113 |
| <I/σ(I)> | 29.6 | |
| Completeness [%] | 99.8 | |
| Redundancy | 6.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 293 | 0.1M CHES, ethanol |
