6J98
Crystal structure of P8 from Lactobacillus rhamnosus
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PHOTON FACTORY BEAMLINE BL-1A |
| Synchrotron site | Photon Factory |
| Beamline | BL-1A |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2016-12-17 |
| Detector | DECTRIS EIGER X 4M |
| Wavelength(s) | 0.9738 |
| Spacegroup name | P 43 21 2 |
| Unit cell lengths | 40.860, 40.860, 107.010 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 38.170 - 1.560 |
| R-factor | 0.17862 |
| Rwork | 0.178 |
| R-free | 0.19622 |
| Structure solution method | SAD |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.781 |
| Data reduction software | MOSFLM |
| Data scaling software | Aimless |
| Phasing software | AutoSol |
| Refinement software | REFMAC (5.8.0238) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 38.170 | 1.601 |
| High resolution limit [Å] | 1.560 | 1.560 |
| Rmerge | 0.111 | 0.600 |
| Rpim | 0.180 | |
| Number of reflections | 12894 | 925 |
| <I/σ(I)> | 4.95 | |
| Completeness [%] | 99.7 | 98.98 |
| Redundancy | 20 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | MICROBATCH | 290 | 0.05M Zinc Acetate dihydrate, 20% PEG3350, 5% n-dodecyl-N, N-dimethylamine-N-oxide |
