6J8L

Phytophthora sojae effector PsAvh240 inhibits a host aspartic protease secretion to promote infection

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL17U1
Synchrotron site	SSRF
Beamline	BL17U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2016-01-18
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.9785
Spacegroup name	P 21 21 21
Unit cell lengths	35.350, 65.679, 143.199
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	34.320 - 2.300
R-factor	0.213
Rwork	0.211
R-free	0.25160
Structure solution method	MAD
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHENIX
Refinement software	PHENIX (1.13_2998)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	10.000	2.340
High resolution limit [Å]	2.300	6.240	2.300
Rmerge	0.106	0.069	0.800
Rmeas	0.116	0.076	0.893
Rpim	0.046	0.031	0.376
Number of reflections	15483	881	782
<I/σ(I)>	4.8
Completeness [%]	99.7	99.1	99.7
Redundancy	6.3	5.7	5.4
CC(1/2)		0.996	0.717

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION	4	277	20% PEG3350, 0.1 M sodium acetate (pH 4.0), and 0.2 M lithium sulfate