6J7Z

Human mitochondrial Oligoribonuclease in complex with RNA

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSRRC BEAMLINE BL15A1
Synchrotron site	NSRRC
Beamline	BL15A1
Temperature [K]	100
Detector technology	CCD
Collection date	2016-09-11
Detector	RAYONIX MX300HE
Wavelength(s)	1.0
Spacegroup name	C 2 2 21
Unit cell lengths	86.192, 125.892, 86.008
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	27.405 - 2.005
R-factor	0.2072
Rwork	0.206
R-free	0.22770
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2igi
Data reduction software	HKL-2000
Data scaling software	SCALEPACK
Phasing software	PHENIX
Refinement software	PHENIX (1.13_2998)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	2.030
High resolution limit [Å]	2.000	5.430	2.000
Rmerge	0.076	0.046	0.508
Rmeas	0.083	0.050	0.549
Rpim	0.033	0.020	0.208
Number of reflections	31548	1604	1540
<I/σ(I)>	17.1
Completeness [%]	99.6	94.2	100
Redundancy	6.5	5.6	6.9
CC(1/2)		0.997	0.926

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		298.15	0.2 M Ammonium citrate dibasic, 20% w/v PEG 3350