6J7Y

Human mitochondrial Oligoribonuclease in complex with DNA

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSRRC BEAMLINE BL15A1
Synchrotron site	NSRRC
Beamline	BL15A1
Temperature [K]	100
Detector technology	CCD
Collection date	2016-03-29
Detector	RAYONIX MX300HE
Wavelength(s)	1.0
Spacegroup name	C 2 2 21
Unit cell lengths	87.282, 125.293, 85.478
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	28.340 - 2.203
R-factor	0.2021
Rwork	0.198
R-free	0.24380
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2igi
Data reduction software	HKL-2000
Data scaling software	SCALEPACK
Phasing software	PHENIX
Refinement software	PHENIX (1.13_2998)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	2.240
High resolution limit [Å]	2.200	5.970	2.200
Rmerge	0.079	0.029	0.565
Number of reflections	23925	1292	1172
<I/σ(I)>	13.7
Completeness [%]	99.3	98.5	98.6
Redundancy	6.7	6.2	6.8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		298.15	0.2 M Ammonium citrate dibasic, 20% w/v PEG 3350