6J7M

Complex structure of the Pseudomonas aeruginosa rhamnosyltransferase EarP with the acceptor elongation factor EF-P

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL19U1
Synchrotron site	SSRF
Beamline	BL19U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2016-12-22
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.988
Spacegroup name	C 1 2 1
Unit cell lengths	223.452, 56.736, 132.419
Unit cell angles	90.00, 125.03, 90.00

Refinement procedure

Resolution	38.041 - 2.301
R-factor	0.1907
Rwork	0.188
R-free	0.24160
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6j7j
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX (1.14_3260)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	40.000	40.000	2.380
High resolution limit [Å]	2.300	4.950	2.300
Rmerge	0.113	0.072	0.468
Rmeas	0.130	0.083	0.541
Rpim	0.063	0.040	0.266
Total number of observations	231937
Number of reflections	59438	6121	5872
<I/σ(I)>	4.8
Completeness [%]	98.6	98.1	98
Redundancy	3.9	4	3.8
CC(1/2)		0.992	0.822

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293	0.2M Ammonium sulfate, 0.1 M MES pH 6.5, 20% PEG8K