6J7I

Fusion protein of heme oxygenase-1 and NADPH cytochrome P450 reductase (15aa)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SPRING-8 BEAMLINE BL44XU
Synchrotron site	SPring-8
Beamline	BL44XU
Temperature [K]	100
Detector technology	CCD
Collection date	2016-12-14
Detector	RAYONIX MX225HE
Wavelength(s)	0.9
Spacegroup name	P 21 21 21
Unit cell lengths	82.771, 158.101, 188.515
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	40.037 - 3.300
R-factor	0.1952
Rwork	0.192
R-free	0.24060
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6j79
RMSD bond length	0.003
RMSD bond angle	0.727
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	MOLREP
Refinement software	PHENIX ((1.13_2998: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	3.360
High resolution limit [Å]	3.300	3.300
Number of reflections	35507	1673
<I/σ(I)>	8.2	1.97
Completeness [%]	91.6	88.6
Redundancy	3.3
CC(1/2)		0.707

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	6	293	PEG 20000, MES-NaOH, ethyl acetate