6J7F
Complex of GGTaseIII, farnesyl-Ykt6 (C-terminal methylated), and GGPP
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL41XU |
| Synchrotron site | SPring-8 |
| Beamline | BL41XU |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2018-04-12 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 1 |
| Spacegroup name | P 41 21 2 |
| Unit cell lengths | 119.415, 119.415, 210.649 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 48.185 - 2.883 |
| R-factor | 0.2079 |
| Rwork | 0.206 |
| R-free | 0.24940 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 6j6x |
| RMSD bond length | 0.002 |
| RMSD bond angle | 0.500 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.930 |
| High resolution limit [Å] | 2.880 | 2.880 |
| Number of reflections | 35109 | 1721 |
| <I/σ(I)> | 12.3 | 2.1 |
| Completeness [%] | 99.9 | 99.9 |
| Redundancy | 16.2 | 9.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 293 | 1M Ammonium Tartrate dibasic pH 7 |
