6J7B

Crystal structure of VASH1-SVBP in complex with epoY

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL18U1
Synchrotron site	SSRF
Beamline	BL18U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2018-11-12
Detector	DECTRIS PILATUS3 S 6M
Wavelength(s)	0.97853
Spacegroup name	P 2 21 21
Unit cell lengths	44.310, 70.920, 128.130
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	47.540 - 1.618
R-factor	0.1789
Rwork	0.178
R-free	0.19700
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6j4o
RMSD bond length	0.007
RMSD bond angle	0.904
Data reduction software	iMOSFLM
Data scaling software	pointless
Phasing software	PHASER
Refinement software	PHENIX ((1.12_2829: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	70.920	1.710
High resolution limit [Å]	1.620	1.620
Rmerge		1.326
Rpim	0.039	0.390
Number of reflections	52501	7575
<I/σ(I)>	12.8
Completeness [%]	100.0
Redundancy	12
CC(1/2)	0.998	0.686

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	EVAPORATION	6	293	20%PEG6000, 1M lithium chloride, 0.1M MES pH6.0